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Abstract: This paper presents a region of interest based on medical images (ROI) of the blind detection watermarking algorithm will contain important pathological information of gray image as digital watermark, after scrambling after the chaotic sequence embedded into the host under the control of wavelet-domain Non-interest area. The experimental results show that the algorithm in the protection of medical ROI while increasing watermark robustness against JPEG compression, and tampering, cropping, filtering Dengjun has good robustness and transparency, but also enhanced the system chaotic sequence security.