Introduction - If you have any usage issues, please Google them yourself
FCMTHRESH Thresholding by 3-class fuzzy c-means clustering
[bw,level]=fcmthresh(IM,sw) outputs the binary image bw and threshold level of image IM using a 3-class fuzzy c-means clustering. It often works better than Otsu s methold which outputs larger or smaller threshold on fluorescence images.