Input original image to strike a gradient image of the gradient image make prospects mark that each center of the target input image to make a Marker (can be manually selected, can also be used to obtain threshold method), the gradient image as a local with the Marker pole mark lower value, and then convert the waters, the waters can be obtained based on marker segmentation results

watershed segmentation\cells.tif
......................\watershed segmentation.txt
watershed segmentation

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