Description: Reverse transcription(RT) followed by PCR is a powerful tool for the detection and quantification of mRNA. It is the most sensitive method for the detection and quantification of gene expression levels, in particular for low abundance mRNA.
The relative quantification is based on the expression ratio of a target gene versus a reference gene. Some mathematical models have already been developed to calculate the relative expression ratios, with or without efficiency correction. Normally the PCR efficiency is set at 2 (the max possible value) for the reference and target gene, but a difference in PCR efficiency of 0.03 between the target and reference gene, the falsely calculated difference in expression ratio is 46 in case of Et<Er and 209 in the case of Et>Er. The difference will increase dramatically by higher efficiency differences: i.e. DE=0.05 (27 and 338 ) and DE=0.1 (7.2 and 1083 )
This function computes the efficiency of PCR reaction and is based on my function MYREGR
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license.txt
pcreff.m
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