cntrd: calculates the centroid of bright spots to sub-pixel accuracy. Inspired by Grier & Crocker s feature for IDL, but greatly simplified and optimized for matlab INPUTS: im: image to process, particle should be bright spots on dark background with little noise ofen an bandpass filtered brightfield image or a nice fluorescent image mx: locations of local maxima to pixel-level accuracy from pkfnd.m sz: diamter of the window over which to average to calculate the centroid. should be big enough to capture the whole particle but not so big that it captures others. if initial guess of center (from pkfnd) is far from the centroid, the window will need to be larger than the particle size. RECCOMMENDED size is the long lengthscale used in bpass plus 2. interactive: OPTIONAL INPUT set this variable to one and it will show you the image used to calculate each centroid, the pixel-level peak and the centroid

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