Using the modified registry, using the drive file exclusive mode, disable and enable the USB port.

DisableUSB\DisableUSB\App.config
DisableUSB\DisableUSB\bin\Debug\DisableUSB.exe
DisableUSB\DisableUSB\bin\Debug\DisableUSB.exe.config
DisableUSB\DisableUSB\bin\Debug\DisableUSB.pdb
DisableUSB\DisableUSB\bin\Debug\DisableUSB.vshost.exe
DisableUSB\DisableUSB\bin\Debug\DisableUSB.vshost.exe.config
DisableUSB\DisableUSB\bin\Debug\DisableUSB.vshost.exe.manifest
DisableUSB\DisableUSB\DisableUSB.csproj
DisableUSB\DisableUSB\Form1.cs
DisableUSB\DisableUSB\Form1.Designer.cs
DisableUSB\DisableUSB\Form1.resx
DisableUSB\DisableUSB\obj\Debug\DesignTimeResolveAssemblyReferences.cache
DisableUSB\DisableUSB\obj\Debug\DesignTimeResolveAssemblyReferencesInput.cache
DisableUSB\DisableUSB\obj\Debug\DisableUSB.csproj.FileListAbsolute.txt
DisableUSB\DisableUSB\obj\Debug\DisableUSB.csproj.GenerateResource.Cache
DisableUSB\DisableUSB\obj\Debug\DisableUSB.csprojResolveAssemblyReference.cache
DisableUSB\DisableUSB\obj\Debug\DisableUSB.exe
DisableUSB\DisableUSB\obj\Debug\DisableUSB.Form1.resources
DisableUSB\DisableUSB\obj\Debug\DisableUSB.pdb
DisableUSB\DisableUSB\obj\Debug\DisableUSB.Properties.Resources.resources
DisableUSB\DisableUSB\obj\Debug\TemporaryGeneratedFile_036C0B5B-1481-4323-8D20-8F5ADCB23D92.cs
DisableUSB\DisableUSB\obj\Debug\TemporaryGeneratedFile_5937a670-0e60-4077-877b-f7221da3dda1.cs
DisableUSB\DisableUSB\obj\Debug\TemporaryGeneratedFile_E7A71F73-0F8D-4B9B-B56E-8E70B10BC5D3.cs
DisableUSB\DisableUSB\Program.cs
DisableUSB\DisableUSB\Properties\AssemblyInfo.cs
DisableUSB\DisableUSB\Properties\Resources.Designer.cs
DisableUSB\DisableUSB\Properties\Resources.resx
DisableUSB\DisableUSB\Properties\Settings.Designer.cs
DisableUSB\DisableUSB\Properties\Settings.settings
DisableUSB\DisableUSB.sln
DisableUSB\DisableUSB.v12.suo
DisableUSB\DisableUSB\obj\Debug\TempPE
DisableUSB\DisableUSB\bin\Debug
DisableUSB\DisableUSB\obj\Debug
DisableUSB\DisableUSB\bin
DisableUSB\DisableUSB\obj
DisableUSB\DisableUSB\Properties
DisableUSB\DisableUSB
DisableUSB

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